Cell Biology FacilityBACK

Laser Scanning Confocal Microscope Zesis 730 3-channel

Instrument information

1. Provider: Carl Zeiss

2. Model:  LSM 710

3, Charge: 100Yuan/h


Specifications

Laser Unit:

·         LD laser: 405 nm

·         LD (R) laser: 635 nm

·         Multi Ar laser: 458 nm, 488 nm, 514 nm

·         HeNe laser: 594 nm


Scanning Unit

1.    Confocal detector

      Confocal detector

a. Detector: 3 simultaneous confocal fluorescence channels with highly sensitive、 low dark noise PMTs.

b. Wavelength resolution: 3 nm;

·         Scanning modes

a.Pixel size: 4×1—6144×6144 pixels

b. Scanning speed: 15×2 speed stages; up to 8 frames/s with 512×512 pixels (max.250 frames/s 512×16); up to 4000 lines per second

c. Scan zoom: 0.6× to 40×; digital variable in steps of 0.1

d.  Scan Field: 20 mm field diagonal(max.) in the intermediate plan, with full pupil illumination

2.    Z-drive

Minimum increment: 25 nm

3.    Microscope

Inverted microscopes

Objects: 10×、20×、40×;63×、100×(Oil);

4.    Software

ZEN 2010


Main features and Service

Laser Scanning Confocal Microscopy (LSCM) is proving to be a most important technique in morphological analyses, molecular and cellular biology, neuroscience and pharmacology. LSCM use lasers as polarized light sources of specified wavelengths, combine with scanning devices, conjugate focusing devices, and computer-controlled image digitizing and analyzing softwares, LSCM enables researchers to collect fluorescent signals from the interior of cells or organisms with improved depth discrimination and resolution. With LSCM, researchers can:

1.      observe very small structures within a cell or an organism and follow their morphological changes;

2.      optically slice a sample and then reconstruct and analyze its three-dimensional structure;

3.      record concentration change of important ions or pH changes at the sub-cellular level,

4.      in combination with electrophysiological methods, study the physiological activity of cells.

The ZEISS LSM710 3-channel can collect fluorescence signals of different wavelengths simultaneously through its 3 channels, which consists of a light diffracting device and 34 highly sensitive photomultiplier tubes. With the integrated Emission Fingerprinting function, widely overlapping emission spectra can be precisely separated, fluorescence bleed-through can be mostly eliminated, and loss of fluorescence signal can be reduced to a minimum. The LSM710 3-channel provides a flexible means for life science research, and is suitable for newly emergent fluorescence applications such as simultaneous intracellular tracking of multiple fluorescent proteins, as well as FRAP and FRET experiments.


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